Abstract:【Objectives】 To summarize the management of cardiopulmonary bypass (CPB) with totally video-assisted thoracoscopy in heart operation. 【Methods】 The CPB established methods, CPB process and postoperative results of 110 patients undergoing operation with totally video-assisted thoracoscopy were analyzed. The surgical procedures included 79 cases of atria septal defect repair (25 concomitant tricuspid valve plasty), 16 ventricular septal defect repair, 4 partia atrioventricular canal defect repair,10 mitral valve replacement and 1 triatriatum defect repair. 【Results】 110 cases CPB time was 61~364 (133±64)min, the aortic cross-clamping time was 17~192 (82±45)min, post-operation respiratory auxiliary time was 4.0~26.5 (4.5±1.8)h, post-operation hospitalization duration was 5~10 days, and 92 cases did not use blood products (83.6%). One patient was not fully heparinization during CPB intubation, and replaced the femoral artery and venous intubation before CPB. Retroperitoneal oma was caused in one patient by CPB intubation. All patients recovered well and discharged uneventfully. 【Conclusion】 Heart operations can be done by applying cardiopulmonary bypass with totally video-assisted thoracoscopy. The method is safe and practicable. But it should be modified to shorten the duration of CPB and aorta clamping.