Abstract:【Objective】 To evaluate the safety and technical feasibility of uniportal complete video-assisted thoracoscopic (cVATS) segmentectomy. 【Methods】 From December 2013 to April 2014, 10 patients underwent uniportal cVATS segmentectomy including 8 men and 2 women, with a median age 57 (39~71) years. 1 case underwent right upper lobectomy and right lower superior segmentectomy. The perioperative data were collected and reviewed. 【Results】 9 cases underwent uniportal cVATS segementectomy uneventfully. 1 case transferred to lobectomy. No conversion to biportal, triportal or open surgery. Mean surgical time was recorded as (156.1±51) (90~240) min and mean blood loss (49.5±57) (5~200) mL. Mean number of lymph nodes was (14.8±4.1) (10~19) and mean number of explored stations was (5.6±0.89) (5~7). Overall, the median duration of chest-tube placement was (2.5±1.4) (1~6)days, and median length of stay was (5.4±2.3) (3~10) days. In 1 case the reserved lung expanded inadequately postoperatively, which was solved by vacuum suction. No postoperative severe complications and death occurred. Lung cancer patients were followed up for 1-5 months without recurrence or metastasis. 【Conclusion】 Uniportal cVATS segmentectomy is a safe, feasible and reproducible surgical procedure with less postoperative pain.