【Objective】 To observe the location of femoral tunnel and functional prognosis of anatomic anterior cruciate ligament (ACL) reconstruction aided by three-dimensional CT (3D-CT). 【Methods】 During the period between Jan. 2011 and Jan. 2012, 16 patients suffered from ACL rapture were treated by arthroscopic autograft ACL reconstruction aided by three-dimensional CT. The average age of the patients was (32±11) years. Patients suffered from knee problem average (41.4±46.1) months before operation. They were followed up post-operationally average (16.7±3.5) months. After operation, 3D-CT analysis of patients were performed to show the location of femoral insertion. The patients were assessed pre-operationally and post-operationally by the Lysholm score. 【Results】 Post-operational 3D-CT showed anatomic location of femoral insertion of ACL reconstruction, which was close to the midpoint between native AM and PL bundles of ACL. Lysholm score of patients showed a significant improvement from (66.9±6.4) pre-operation to (83.5±5.3) post-operation (t =8.25, P <0.001). 【Conclusion】 Application of 3D-CT can precisely identify the location of femoral insertion of ACL reconstruction, which is helpful for surgeon to perform anatomic reconstruction and for patients to obtain excellent prognosis.