Abstract:【Objective】 To evaluate the clinical efficacy and value of laparoendoscopic single-site surgery (LESS) in retrospective or conventional laparoscopic retroperitoneal adrenalectomy. 【Methods】 68 patients underwent LESS retroperitoneal adrenalectomy (Group A), another 74 patients with benign adrenal tumors served as a conventional multiport laparoscopic retroperitoneal adrenalectomy(Group B). The clinical intraoperative, postoperative data were compared retrospectively between the two groups. 【Results】 There were significant differences between the two groups in terms of length of incision, time to anal exsufflation, cases of postoperation analgesia, postoperative pain verbal rating scale (VRS), time to ambulation(P <0.05); no significant differences between the two groups in terms of the operative time, intraoperative estimated blood loss, perioperative complications, drainage volume, time of postoperative drainagea, postoperative hospital stay(P >0.05). 【Conclusion】 Retroperitoneal laparoendoscopic single-site surgery(LESS) adrenalectomy is a safe and effective procedure, which presents a better cosmetic outcome, less postoperative pain and quicker postoperative recovery based on the extensive experience with conventional laparocopic skill,which helps to reduce the operation time, shorten the learning curve.