Abstract:【Objective】 To evaluate the feasibility of closing T-tube instantly following laparoscopic common bile duct exploration,and explore the relationship between postoperative biliary leak and intraoperative closing T-tube instantly. 【Methods】 152 patients were closed T-tube instantly following laparoscopic common bile duct exploration and randomly divided into three groups by different common bile duct suture methods with various suture materials. The incidence of intraoperative and postoperative biliary leak were observed in each group, and common bile duct pressure was recorded respectively at the same time. 【Results】 There were 13 cases (26.00%) of bile leakage occurred in 3-0 absorbable thread interrupted suture group, 9 cases (16.98%) in 3-0 absorbable line continuous suture group, and 0 case in 3-0vlock line continuous suture group respectively, comparison of biliary leak rate among three groups were with significant statistical significance (P <0.05). All patients of bile leakage were restoration after simple abdominal cavity drainage and had no extrahepatic bile duct stricture after operation or other serious complications, no death occurred. 【Conclusion】 The approach of intraoperative closing T-tube instantly with 3-0Vlock continuous sutures following laparoscopic choledocholithotomy is safe and feasible, which may effectively avoid the risk of biliary leak caused by increased pressure.