【Objective】 To investigate the hemostatic effect in laparoscopic cholecystectomy with absorbable hemostatic microspheres. 【Methods】 307 cases treated with laparoscopic cholecystectomy were randomly divided into 2 groups, Control group: Traditional hemostatic method was used in the operation to the bleeding. Study group: Absorbable hemostatic microspheres was used in the operation to the bleeding. Success rate of intraoperative bleeding 2 minutes, intraoperative blood loss, postoperative flow were evaluated. 【Results】 The intraoperative blood loss and postoperative flow were significantly little in Study group when compared with Control group (P <0.05). The Success rate of intraoperative bleeding 2 minutes was better in Study group when compared with Control group (P <0.05). 【Conclusion】 Using Absorbable hemostatic microspheres in laparoscopic cholecystectomy has a significant hemostatic effect, it is worth promoting.