Abstract:【Objective】 To investigate long-term efficacy of interbody fusion using single B-Twin ESS through microendoscopic approach for lumbar degenerative disease. 【Methods】 A retrospective analysis was made on data of 21 cases or 22 disc levels underwent fusion using a single B-Twin ESS by a same surgeon from Feb. 2007 to Jan. 2010. The follow-up time ranged from 38 to 64 months and averaged 50 months. JOA were scored preoperatively and postoperatively and the disc height were measured at every follow-up. Healing state of the bone graft in the disc space was evaluated by Suk's criteria. 【Results】 JOA score were (11.6±2.9) preoperatively and (26.6±1.5) at final follow-up, respectively, JOA improvement rate was 64.7%~94.4%, the average was 86.4%, improvement was markedly effective postoperatively in all 21 cases at the final follow-up; The average disc height was (9.5±1.5) mm preoperatively, (11.5±1.3) mm at 1 week postoperatively, (10.0±1.4) mm at 6 months postoperatively and (9.4±1.7) mm at final follow-up, respectively, and the difference between the disc height at 6 months postoperatively and at final follow-up was significant (P >0.05). Solid union occurred in 9 cases (40.9%) and probable union in 13 cases (56.3%), None nonunion case was found by Suk's criteria. 【Conclusion】 The long-term efficacy was satisfied interms of pain relieve for lumbar degenerative disease with fusion using single B-Twin ESS through microendoscopic approach, but the solid fusion rate needs to be improved.