Abstract:【Objective】 To explore the feasibility of endoscopy in etiological diagnosis of difficult in ascites. 【Methods】 The protocol went as following: paraumbilical left 2 cm puncture, local infiltration anesthesia small incision, skin, artificial pneumoperitoneum, insert the trocar, through trocar into strict disinfection of gastroscope, according to the right, left, right, left lower sequential observation, lesions finding, biopsy forceps biopsy of insertion; abdominal hemorrhage may be RF direct burning of hemostasis, intraperitoneal adhesion belt can be RF burning off. 【Results】 In 110 patients with refractory ascites, 104 cases achieved positive results, the positive rate was 94.5%, of which 75 cases of tuberculous peritonitis (72%), peritoneal metastatic carcinoma in 15 cases (14.4%), 4 cases of malignant lymphoma (3.8%), 3 cases of ovarian carcinoma and ascites, liver and peritoneal metastasis in 3 cases, 4 cases of other diseases, 6 cases of visceral, parietal peritoneum were not abnormal. Adverse reaction: this group of 110 cases on behalf of laparoscopy, 3 cases of mild postoperative fever, 1 case of severe adhesion caused by intestinal perforation. 【Conclusion】 Electronic gastroscope diagnosis of ascites with safe, reliable, simple operation, less complications, is worthy of popularization and application.