Abstract:【Objective】 To explore the clinical application of endotoscope in repairing the perforation of tympanic membrane in primary hospital. 【Methods】 We analyzed the data from 54 patients operated on for a tympanic perforation using endotoscope with different graft. 【Results】 Patients were followed up postoperatively for 3~6 months. The average closure rate of tympanic membrane perforations was 88.9% (92.3% in fat group, 90% in temporal fascia group, 88.46% in perichondrium group and 80% in perichondrial cartilage group). The improvement in pure tone air conduction threshold more than 10 dBHL was achieved in 38 cases after operation, the difference was statistically significant (P <0.05). 【Conclusion】 Endotoscopic myringoplasty is a rapid, safe, reliable and efficient procedure that should be performed in primary hospital. Different graft material should be chosen reasonably according to different size and type of the tympanic membrane perforation.