Abstract:【Objective】 To explore the safety and feasibility of single-port anatomical retroperitoneal laparoscopic adrenalectomy by using a self-made port. 【Methods】 From January 2009 to November 2012, single-port anatomical retroperitoneal laparoscopic adrenalectomy was performed by using a self-made port in 21 patients (14 men and 7 women) with adrenal tumor. The mean patient age was 43(19~56) years. The mean body mass index was 23.4 kg/m2. 【Results】21 cases were completed successfully without conversion to open or standard laparoscopic surgery. The mean operative time was (92.8±21.6) min, the mean blood loss was (64.3±11.3) mL, no major complication occurred during surgery. The post-operative pain score (VAPS) in the first post-operative day was 0~3, none of the patients received analgesic. The mean post-operative hospital stay was 4 days, No post-operative complications was observed after a short-term follow-up. The serum potassium level of patients with aldosterone-producing adenoma were returned to normal range, the blood pressure in 2 patients after surgery was still higher than normal, but needed less antihypertensive medications compared with pre-operation. 【Conclusion】 The self-made triport in the single-site anatomical retroperitoneal laparoscopic adrenalectomy is safe and feasible, and is of a fine clinical application prospect. However, these results should be further proved by large-scale random control trials.