血清生长抑制蛋白家族成员5水平与早期胃癌患者内镜黏膜下剥离术后复发的关系

Relationship between serum ING5 levels and recurrence after endoscopic submucosal dissection in early gastric cancer patients

摘要:

目的 探讨血清生长抑制蛋白家族成员5（ING5）表达水平与早期胃癌患者内镜黏膜下剥离术（ESD）后复发的关系。方法 选取2017年5月－2019年5月于该院行ESD的早期胃癌患者96例（研究组），另选择同期该院健康体检者96例（对照组）。采用实时荧光定量聚合酶链反应（qRT-PCR）检测ING5表达水平。通过多因素Logistic回归模型，分析影响早期胃癌患者ESD术后复发的因素。结果 研究组血清ING5表达水平明显低于对照组，差异有统计学意义（P < 0.05）；血清ING5表达水平与分化程度和浸润深度有关（P < 0.05）；早期胃癌患者ESD术后，复发组癌组织为中分化和浸润深度为黏膜下层比例明显高于未复发组（P < 0.05）；ING5 < 0.75、分化程度为中分化和浸润深度为黏膜下层，是影响早期胃癌患者ESD术后复发的独立危险因素（P < 0.05）。结论 ING5在早期胃癌患者的血清中呈低表达，而且是ESD术后复发的影响因素之一，其可作为一种抑癌基因标志物应用于临床研究中。

Abstract:

Objective To explore the relationship between serum inhibitor of growth family member 5 (ING5) expression levels and postoperative recurrence after endoscopic submucosal dissection (ESD) in early gastric cancer patients.Methods 96 patients of early gastric cancer (study group) who underwent ESD surgery from May 2017 to May 2019, while 96 healthy patients individuals who underwent physical examinations during the same period (control group). Real time fluorescent quantitative polymerase chain reaction (qRT-PCR) was used to detect the ING5 expression level. Analysis of influencing factors through multivariate Logistic regression.Results The serum ING5 expression level in the study group was significantly lower than that in the control group (P < 0.05); The serum ING5 expression level was related to the degree of differentiation and depth of infiltration (P < 0.05); The proportion of moderate differentiation and submucosal infiltration depth in gastric cancer tissue in the recurrent group of gastric cancer patients after ESD surgery was significantly higher than those in the non recurrent group (P < 0.05); ING5 < 0.75, moderate differentiation and submucosal infiltration depth were independent risk factors for postoperative recurrence in early gastric cancer patients undergoing ESD surgery (P < 0.05).Conclusion The expression of ING5 is down-regulated in the serum of patients with early gastric cancer, and is an influencing factor of postoperative recurrence of ESD. It can be used as a tumor suppressor gene marker in clinical research.