血清微RNA-198和微RNA-365联合胃镜检查在慢性萎缩性胃炎诊断中的应用效果

Clinical application of serum microRNA-198 and microRNA-365 combined with gastroscopy in diagnosis of chronic atrophic gastritis

摘要:

目的 分析血清微RNA-198（miRNA-198）和微RNA-365（miRNA-365）联合胃镜检查在慢性萎缩性胃炎（CAG）诊断中的价值。方法 选取2022年1月－2023年1月在该院行胃镜检查的患者360例作为研究对象。其中，180例CAG患者为观察组，同期180例慢性非萎缩性胃炎患者作为对照组。根据CAG患者萎缩程度分为：轻度56例，中度84例，重度40例。患者均经胃镜检查，并采用实时荧光定量聚合酶链反应（qRT-PCR）法，对血清中miRNA-198和miRNA-365的相对表达水平进行检测。绘制受试者操作特征曲线（ROC curve），分析血清miRNA-198和miRNA-365水平对CAG的诊断价值。采用四格表，分析血清miRNA-198和miRNA-365联合胃镜检查对CAG的诊断价值。结果 与对照组相比，观察组血清miRNA-198和miRNA-365水平明显降低，且随着萎缩程度的加重，血清miRNA-198和miRNA-365水平依次降低，差异均有统计学意义（P < 0.05）；血清miRNA-198和miRNA-365水平诊断CAG的曲线下面积（AUC）分别为0.898和0.945，联合胃镜检查诊断CAG的敏感度为98.33%，特异度为81.11%，准确度为89.72%，其诊断效能均高于血清miRNA-198、miRNA-365和胃镜单独诊断。结论 CAG患者血清miRNA-198和miRNA-365水平降低，对CAG具有一定的诊断价值，联合胃镜检查，可明显提高CAG的诊断价值。

Abstract:

Objective To analyze the value of serum microRNA-198 (miRNA-198) and miRNA-365 combined with gastroscopy in diagnosis of chronic atrophic gastritis (CAG).Methods 180 patients with CAG who were treated from January 2022 to January 2023 were regarded as the observation group, and 180 patients with chronic non-atrophic gastritis in the same period were studied as control group. According to the degree of atrophy, patients with CAG were grouped into 56 mild cases, 84 moderate cases and 40 severe cases. The subjects were examined by gastroscopy, and the relative expression levels of miRNA-198 and miRNA-365 in serum were detected by real-time fluorescent quantitative polymerase chain reaction (qRT-PCR). The diagnostic value of serum miRNA-198 and miRNA-365 levels in patients with CAG was analyzed by receiver operator characteristic curve (ROC curve). The diagnostic value of serum miRNA-198 and miRNA-365 combined with gastroscopy for CAG was analyzed with four grid tables.Results Compared with the control group, the levels of serum miRNA-198 and miRNA-365 in patients with CAG in the observation group were obviously lower, with the aggravation of atrophy, the levels of serum miRNA-198 and miRNA-365 decreased in turn, the differences were statistically significant (P < 0.05); the area under the curve (AUC) of serum miRNA-198 and miRNA-365 levels in the diagnosis of CAG was 0.898 and 0.945, respectively, the sensitivity, specificity and accuracy of combined gastroscopy in the diagnosis of CAG were 98.33%, 81.11% and 89.72%, respectively, its diagnostic efficacy was higher than that of serum miRNA-198, miRNA-365 and gastroscopy alone.Conclusion The levels of serum miRNA-198 and miRNA-365 in patients with CAG are decreased, they have certain diagnostic value for CAG, their combination with gastroscopy can obviously improve the diagnostic value of CAG.