血清miR-505和miR-15与溃疡性结肠炎内镜下活动度的关系

Relationship between serum miR-505, miR-15 and endoscopic activity of ulcerative colitis

摘要:

目的 分析血清miR-505和miR-15与溃疡性结肠炎（UC）内镜活动度的关系。方法 选取2020年5月－2022年5月该院收治的98例UC患者作为观察组，并选取同期80例肠易激综合征（IBS）患者作为对照组。采用改良Mayo评分，评估UC患者的内镜活动度，分析血清miR-505和miR-15水平变化与UC患者内镜活动度的关系。结果 观察组血清miR-505水平低于对照组，miR-15水平高于对照组，差异均有统计学意义（P < 0.05）。活动期UC患者血清miR-505水平低于愈合期患者，血清miR-15水平高于愈合期患者，差异均有统计学意义（P < 0.05）。E3型血清miR-505水平低于E2型和E1型，E3型血清miR-15水平高于E2型和E1型，E3型改良Mayo评分高于E2型和E1型，差异均有统计学意义（P < 0.05）。血清miR-505与改良Mayo评分呈负相关（r = -0.51，P < 0.05），血清miR-15与改良Mayo评分呈正相关（r = 0.54，P < 0.05）。受试者操作特征曲线（ROC curve）结果显示，miR-505截断值为0.93，评估UC患者内镜活动度的曲线下面积（AUC）为0.67（95%CI：0.659～0.785），敏感度和特异度分别为73.24%和68.55%；miR-15截断值为0.85，评估UC患者内镜活动度的AUC为0.69（95%CI：0.672～0.814），敏感度和特异度分别为76.38%和69.72%；两者联合评估UC患者内镜活动度的AUC为0.82（95%CI：0.809～0.912），敏感度和特异度分别为84.56%和68.49%。结论 UC患者血清miR-505水平较IBS患者降低，血清miR-15较IBS患者升高，其水平与患者病变情况及内镜下活动度密切相关，血清miR-505和miR-15联合检测对UC患者内镜下活动度具有较好的评估价值。

Abstract:

Objective To investigate the relationship between serum miR-505, miR-15 and endoscopic activity of ulcerative colitis (UC).Methods 98 patients with UC from May 2020 to May 2022 were selected as the observation group, and 80 patients with irritable bowel syndrome (IBS) were selected as the control group during the same period. The modified Mayo score was used to evaluate the endoscopic activity in patients with UC, and the relationship among the changes of serum miR-505 and miR-15 levels and the endoscopic activity in patients with UC was analyzed.Results The level of serum miR-505 in observation group was lower than that in control group, while the level of miR-15 in observation group was higher than that in control group (P < 0.05). The level of serum miR-505 in patients with active UC was lower than that in patients with the healing stage, while the level of serum miR-15 in patients with active UC was higher than that in patients with healing stage (P < 0.05). The serum miR-505 level of E3 type was lower than that of E2 type and E1 type, and the serum miR-15 level of E3 type was higher than that of E2 type and E1 type (P < 0.05). The Mayo score of type E3 was higher than that of type E2 and E1 (P < 0.05). Pearson correlation analysis showed that serum miR-505 was negatively correlated with Mayo score（r = -0.51, P < 0.05）, while serum miR-15 was positively correlated with Mayo score (r = 0.54, P < 0.05). Receiver operator characteristic curve (ROC curve) results showed that the cut-off value of miR-505 was 0.93, and the area under the curve (AUC) for evaluating the endoscopic activity of patients with UC was 0.67 (95%CI: 0.659 ~ 0.785), and the sensitivity and specificity were 73.24% and 68.55%, respectively. The cut-off value of miR-15 was 0.85, and the AUC for evaluating the endoscopic activity of patients with UC was 0.69 (95%CI: 0.672 ~ 0.814), and the sensitivity and specificity were 76.38% and 69.72%, respectively. The AUC for the joint evaluation of UC by both was 0.82 (95%CI: 0.809 ~ 0.912), and the sensitivity and specificity were 84.56% and 68.49%, respectively.Conclusion Serum miR-505 levels decrease and serum miR-15 levels increase in UC patients than IBS, and the level is closely related to patients’ pathological conditions and endoscopic activity. Combined detection of serum miR-505 and miR-15 has a good value in evaluating endoscopic activity in UC patients.