支气管内光学相干断层成像技术评估兔创伤性气道狭窄类型的诊断价值

Diagnostic value of EB-OCT in evaluating the type of airway stenosis in traumatized rabbits

摘要:

目的 使用支气管内光学相干断层成像技术（EB-OCT）获得兔创伤性气道狭窄模型的图像，评估EB-OCT在判断良性气道狭窄类型中的诊断价值。方法 建立黏膜破坏联合软骨破坏的兔创伤性气道狭窄模型，建模成功12只，获取12只气道狭窄兔术后2、4、6和8周支气管镜、EB-OCT图像特点和气管组织病理学图像，测量EB-OCT和病理图像匹配部分软骨层及非软骨层的厚度，并比较两者的相关性。结果 12只建模成功的气道狭窄兔的EB-OCT图像特点：低信号反射的软骨层结构，从断裂、不连续到软骨膜逐渐修复；EB-OCT诊断气管结构层次：黏膜破坏3例，黏膜破坏及软骨破坏9例，与组织病理学比较，差异无统计学意义（P > 0.05）。EB-OCT和病理图像匹配部分软骨层及非软骨层的厚度呈线性正相关，相关曲线分别是：软骨层Y = 3.7498+0.9717X，r = 0.9992（P < 0.01）；非软骨层Y = -9.4328 + 0.9935X，r = 0.9999（P < 0.01）。结论 EB-OCT可以很好地观察到良性气道狭窄中气管黏膜破坏和/或软骨破坏以及两者的破坏程度，具有病理组织学的诊断优势。

Abstract:

Objective To evaluate the diagnostic value of EB-OCT in diagnosis of benign airway stenosis types by use of examining the images of airway stenosis in traumatic rabbits with EB-OCT.Methods A rabbit model of traumatic airway stenosis with cartilage and mucosal destruction was established. The characteristics of EB-OCT images and histopathological images of trachea were obtained at 2, 4, 6 and 8 weeks after operation in 12 rabbits with successful modeling. The thickness of cartilage layer and non-cartilage layer in the matched part of EB-OCT images was measured. We compared the correlation between EB-OCT images of trachea and corresponding histopathological images of stenotic rabbits.Results The characteristics of EB-OCT images in 12 rabbits with airway stenosis was found as follows. The structure of cartilage layer with low signal reflection was from fracture and discontinuity to perichondrium gradually repaired. EB-OCT was used to evaluate the structure of trachea, in which mucosal damage was 3, and mucosal damage combined with cartilage damage was 9. There was no statistically significant difference between the type of airway stenosis observed by EB-OCT and histopathology in rabbits after trauma (P > 0.05). There was a linear positive correlation between the thickness of cartilage layer and non-cartilage layer in EB-OCT and histopathology. The linear regression equations were Y = -9.4328 + 0.9935X, (P < 0.01), Y = 3.7498 + 0.9717X, (P < 0.01), and the correlation coefficients r were 0.9999 and 0.9992, respectively.Conclusion EB-OCT can estimate the destruction of tracheal mucosa and/or cartilage and the degree of them, with the diagnostic advantage of histopathology.