Objective To evaluate the application of SpyGlass DS choledochoscope in intrahepatic bile duct stones.Methods Retrospective analysis was performed on 35 cases of lithotriptic calculus diagnosed and treated with SpyGlass DS choledochoscope from September 2017 to June 2019. The diagnostic rate, success rate of one-off stone extraction and incidence of complications were evaluated.Results The diagnostic rate of intrahepatic bile duct stones by SpyGlass DS choledochoscope was 97.14% (34/35), the success rate of one-off stone extraction was 85.71% (30/35), and the complication rate was 8.57% (3/35).Conclusion SpyGlass DS choledochoscope is a safe and effective endoscopic treatment for intrahepatic bile duct stones, which can improve the diagnosis and treatment rate.