circMTO1表达与miR-3200/PEBP1轴的关系及其预测内镜黏膜下剥离术后复发的价值
作者:
作者单位:

1.贵黔国际医院 消化内科,贵州 贵阳 550018;2.陆军军医大学第一附属医院(西南医院) 消化内科,重庆 400038

作者简介:

李本杰2012年7月-2019年9月在陆军军医大学第一附属医院(西南医院)消化内科从事临床医疗及消化内镜诊疗工作

通讯作者:

周倩,E-mail:qianxiao75573706@163.com;Tel:18623559761

基金项目:

国家重点研发计划课题(No:2017YFC0112305)


Relationship between the expression of circMTO1 and miR-3200/PEBP1 axis and its predictive effect on the recurrence after endoscopic submucosal dissection
Author:
Affiliation:

1.Department of Gastroenterology, Guiqian International General Hospital, Guiyang, Guizhou 550018, China;2.Department of Gastroenterology, the First Hospital affiliated to AMU (Southwest Hospital), Chongqing 400038, China

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
    摘要:

    目的 研究circMTO1表达与miR-3200/磷脂酰乙醇胺结合蛋白1(PEBP1)轴的关系及其预测内镜黏膜下剥离术(ESD)后复发的价值。方法 选择2015年1月-2018年7月在陆军军医大学第一附属医院(西南医院)接受ESD治疗的早期胃癌患者作为研究对象(早期胃癌组),另取同期经胃黏膜活检明确为正常胃黏膜的受试者作为对照组。检测两组circMTO1、miR-3200和PEBP1的表达水平,随访早期胃癌患者的无复发生存情况,并采用Kaplan-Meier曲线分析无复发生存率的差异。结果 早期胃癌组circMTO1和PEBP1表达水平明显低于对照组,miR-3200表达水平明显高于对照组(P < 0.05),且circMTO1与miR-3200呈负相关、与PEBP1呈正相关,miR-3200与PEBP1呈负相关;早期胃癌组中不同肿瘤直径、浸润深度、淋巴结转移情况和脉管癌栓情况的circMTO1、miR-3200和PEBP1表达水平比较,差异均有统计学意义(P < 0.05);Kaplan-Meier曲线分析显示,circMTO1高表达的早期胃癌患者无复发生存率高于circMTO1低表达患者,miR-3200高表达的早期胃癌患者无复发生存率低于miR-3200低表达患者,PEBP1高表达的早期胃癌患者无复发生存率高于PEBP1低表达患者。结论 早期胃癌中circMTO1低表达与病情恶化和ESD术后无复发生存时间缩短有关,靶向miR-3200/PEBP1轴可能是circMTO1参与早期胃癌发生发展的分子机制。

    Abstract:

    Objective To study the relationship between the expression of circMTO1, and miR-3200/PEBP1 axis and its predictive effect on the recurrence after endoscopic submucosal dissection (ESD).Methods Patients with early gastric cancer who received ESD treatment in the First Hospital Affiliated to AMU (Southwest Hospital) from January 2015 to July 2018 were selected as the research subjects (early gastric cancer group), and the patients who received endoscopic gastric mucosal biopsy and confirmed as normal gastric mucosa by pathology were selected as the control group. The expression levels of circMTO1, miR-3200 and PEBP1 in early gastric cancer and normal gastric mucosa were detected. The recurrence free survival of early gastric cancer patients was followed up. The difference of recurrence free survival rate was analyzed by Kaplan-Meier curve.Results The expression levels of circMTO1 and PEBP1 in the early gastric cancer group were significantly lower than those in the control group, and the expression levels of miR-3200 were significantly higher than that in the control group (P < 0.05), and circMTO1 was negatively correlated with miR-3200 and positively correlated with PEBP1, and miR-3200 was negatively correlated with PEBP1; The expression levels of circMTO1, miR-3200 and PEBP1 were significantly different in the early gastric cancer group with different tumor diameter, depth of invasion, lymph node metastasis and vascular tumor thrombus (P < 0.05); Kaplan-Meier curve analysis showed that the recurrence free survival rate of patients with high expression of circMTO1 was higher than that of patients with low expression of circMTO1, the recurrence free survival rate of patients with high expression of miR-3200 was lower than that of patients with low expression of miR-3200, the recurrence free survival rate of patients with high expression of PEBP1 was higher than that of patients with low expression of PEBP1.Conclusion The low expression of circMTO1 in early gastric cancer relates with the deterioration and the shortening of recurrence free survival time after ESD, targeting miR-3200/PEBP1 axis may be the molecular mechanism of circMTO1 participating in the occurrence and development of early gastric cancer.

    表 2 早期胃癌组不同临床病理特征circMTO1、miR-3200和PEBP1表达比较 (x±s)Table 2 Comparison of the expression of circMTO1,miR-3200 and PEBP1 in different clinicopathological characteristics of early gastric cancer group (x±s)
    图1 Western Blot检测早期胃癌组织和正常胃黏膜组织中PEBP1的表达Fig.1 The expression of PEBP1 in early gastric cancer tissue and normal gastric mucosa tissue detected by Western blot
    图2 早期胃癌组circMTO1、miR-3200和PEBP1表达的相关性Fig.2 Correlation of circMTO1,miR-3200 and PEBP1 expression in early gastric cancer group
    图3 无复发生存率与circMTO1、miR-3200和PEBP1表达的关系Fig.3 Relation between relapse-free survival and expression of circMTO1,miR-3200 and PEBP1
    表 1 两组患者circMTO1、miR-3200和PEBP1表达比较 (x±s)Table 1 Comparison of circMTO1、miR-3200 and PEBP1 expression between the two groups (x±s)
    参考文献
    相似文献
    引证文献
引用本文

李本杰,周倩,彭志红. circMTO1表达与miR-3200/PEBP1轴的关系及其预测内镜黏膜下剥离术后复发的价值[J].中国内镜杂志,2021,27(5):7-13

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
历史
  • 收稿日期:2020-07-31
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2021-06-03
二维码
中国内镜杂志声明
关闭