miR-93表达与内镜黏膜下剥离术治疗老年早期胃癌预后的关系及其靶向抑癌基因的生物信息学分析

Relationship between the expression of miR-93 and the prognosis of endoscopic submucosal dissection in the treatment of elderly early gastric cancer and the bioinformatics analysis of its targeted tumor suppressor genes

摘要:

目的 研究miR-93表达与内镜黏膜下剥离术（ESD）治疗老年早期胃癌预后的关系，利用生物信息学分析miR-93的靶向抑癌基因。方法 选择2015年1月－2017年12月在该院接受ESD的老年胃癌患者，收集早期胃癌组织并检测miR-93、PTEN和TIMP2的表达量，随访术后复发情况；另取同期经病理证实为正常胃黏膜的组织作为对照，检测miR-93、PTEN和TIMP2的表达量。结果 早期胃癌组织中miR-93的表达量高于正常胃黏膜组织，PTEN和TIMP2的表达量低于正常胃黏膜组织（P < 0.05），且miR-93的表达量与PTEN和TIMP2的表达量呈负相关，T₂期、低分化和未分化、黏膜下浸润、发生复发的早期胃癌组织中miR-93的表达量高于T₁期、中分化和高分化、黏膜内浸润、未复发的早期胃癌组织，PTEN、TIMP2的表达量明显低于T₁期、中分化和高分化、黏膜内浸润、未复发的早期胃癌组织（P < 0.05）；黏膜下浸润、miR-93表达增多、PTEN及TIMP2表达减少是早期胃癌患者术后复发的危险因素；miR-93高表达的早期胃癌患者未复发率低于miR-93低表达患者；miR-93预测早期胃癌复发的最佳截点为0.72。结论 miR-93表达是早期胃癌患者ESD后复发的危险因素，对术后复发具有预测价值，靶向抑癌基因PTEN、TIMP2是其可能的生物学作用。

Abstract:

Objective To study the relationship between the expression of miR-93 and the prognosis of endoscopic submucosal dissection in the treatment of elderly early gastric cancer and analyze its targeted tumor suppressor genes by bioinformatics.Methods The elderly patients with early gastric cancer who underwent endoscopic submucosal dissection from January 2015 to December 2017 were selected, the expression of miR-93, PTEN, TIMP2 were determined in early gastric cancer tissues, postoperative recurrence was followed-up; Meanwhile, the normal gastric mucosa tissues confirmed by pathology at the same time were taken as the control and the expression of miR-93, PTEN, TIMP2 were detected.Results The expression of miR-93 in early gastric cancer tissue was higher than that in normal gastric mucosa tissue, the expression of PTEN and TIMP2 was lower than that in normal gastric mucosa (P < 0.05) and the expression of miR-93 was negatively correlated with the expression of PTEN and TIMP2, the expression of miR-93 in early gastric cancer with T₂ stage, low differentiation and undifferentiation, submucosal infiltration, recurrence was higher than that in early gastric cancer with T₁ stage, medium differentiation and high differentiation, intramucosal infiltration and non recurrence, the expression of PTEN and TIMP2 was lower than those in early gastric cancer with T₁ stage, medium differentiation and high differentiation, intramucosal infiltration and non recurrence (P < 0.05); Submucosal infiltration, increased expression of miR-93, decreased expression of PTEN and TIMP2 were risk factors for postoperative recurrence of early gastric cancer; the unrecurring rate of early gastric cancer with high expression of miR-93 was lower than that with low expression of miR-93; the best cut-off point for miR-93 to predict the recurrence of early gastric cancer was 0.72.Conclusion The expression of miR-93 is a risk factor for the recurrence of early gastric cancer patients after endoscopic submucosal dissection and has a predictive value for the recurrence. Targeting tumor suppressor genes PTEN and TIMP2 is a possible biological role.