Abstract: Objective?To explore the feasibility of total laparoscopic pancreaticoduodenectomy (LPD) assisted by mixed reality technique.?Methods?A patient with duodenal tumor was selected for total laparoscopic pancreaticoduodenectomy. The abdominal CT was used for three-dimensional reconstruction and surgical planning of the body surface and target lesions of the patients before operation. The mixed reality technology (Mixed Reality, MR) was used to navigate the surgical approach, important anatomical structures and assist in total laparoscopic pancreaticoduodenectomy, and the operation was completed successfully.?Results?The patient recovered smoothly without obvious complications and was discharged 2 weeks after operation.?Conclusion?MR technique can accurately reconstruct and locate the anatomical structure of the surgical site, which is expected to bring more possibilities and prospects for the development of general surgery.