Abstract:Abstract: Objective?To investigate the cleaning and disinfection and microbial contamination in the otorhinolaryngological endoscopy room, analyze the possible links that may cause related problems in the endoscopic diagnosis and treatment, formulate corresponding prevention and treatment measures, optimize evaluation methods, and reduce the possibility of endoscopic nosocomial infection.?Method?The basic conditions of cleaning and disinfection of the otolaryngology endoscopy room were collected and analyzed, and the qualified rate of endoscopic cleaning and disinfection operation of different detection methods were observed. Endoscope disinfection rate and bacteria classification were used. The indicators of 3 months before and after the improvement measures were divided into control group and observation group, and the indicators were analyzed and compared.?Result?Endoscopic cleaning rate index residual protein test positive rate and ATP bioluminescence detect was significantly higher than 10?× magnifier visual inspection with light source, the difference was statistically significant (P?0.05), the 10?× magnifier visual inspection with light sourcer positive detection rate of the observation group and the control group had no significant difference (P?>?0.05). There were statistically significant differences between the two groups in residual protein detection and ATP bioluminescence positive rate. The qualified rate of endoscopic disinfection and the qualified rate of endoscopic disinfection in the observation group were both lower than those in the control group, and the difference was statistically significant (P?0.05). 66 bacteria strains were isolated from 24 specimens.?Conclusion?ATP fluorescence detector is suitable for rapid and reliable detection of endoscopic cleaning effect in otolaryngology endoscopy room, and the cleaning and disinfection process should be standardized to improve the quality evaluation system and prevent nosocomial infection.