Abstract:Abstract: Objective?To evaluate the effect of preoperative autologous blood labeling in laparoscopic colorectal surgery.?Methods?A prospective study was conducted in 60 patients who needed laparoscopic radical resection of colorectal cancer. Enteroscopy was performed one day before operation, and autologous blood marking was performed at 0.5 cm of the lower margin of the tumor. The location of the marker was observed during the operation. Postoperative anatomical specimens to confirm the accuracy of localization. The operation time, success rate and complications were evaluated. The incision margin of the resected specimens was evaluated by pathology after the operation.?Results?In laparoscopic surgery, autologous blood markers were successfully identified in 58 patients, and laparoscopic radical resection of colorectal cancer was successfully completed. The success rate was 96.7%. There were no complications such as bleeding, perforation and fever in all patients. Two patients failed to find the marker points during the operation, and were successfully operated after endoscopy positioning. Surgical procedure was smooth. Postoperative anatomical specimens, all these 60 patients showed accurate location. Two patients had tumors located in splenic curvature. The label was displayed on the dorsal side and no label on the ventral side, which led to unrecognizability during operation. Pathological returns of the specimens showed negative margins.?Conclusion?Subserous autologous blood labeling under enteroscopy provides a safe, simple, economical and effective method for preoperative localization of laparoscopic colorectal surgery, and can become a new alternative method.