小动物内镜在在体成像中的应用研究

Endoscopic imaging of small animals in vivo*

摘要:

目前小鼠肠道病变监测需要处死小鼠后分离肠道组织进行病理学检查。该研究的目的是构建小动物成像平台，实现肠道病变的在体评估。方法 根据电子内镜成像原理搭建小动物成像平台。选取6只Balb-c小鼠分别给予3%葡聚糖硫酸钠（DSS）或蒸馏水自由饮用，监测小鼠生理状况并利用内镜系统观察肠道黏膜损伤。结果 所有小鼠均在麻醉状态下进行内镜检查，进镜深度可达3 cm，且无内镜相关并发症发生。内镜下可见DSS组小鼠肠道病变逐渐加重，而对照组小鼠肠道黏膜无明显改变。结论 该内镜系统可以对肠道黏膜进行清晰成像，实现病变的在体评估及动态监测。

Abstract:

To construct a small animal imaging platform to evaluate intestinal lesions in vivo. Methods A small animal imaging platform was built according to the principle of electronic endoscopic imaging. 6 Balb-c mice were given 3% sodium dextran sulfate or distilled water for free drinking. Physiological status of the mice was monitored and intestinal mucosal injury was observed by endoscope. Results All the mice were underwent endoscopy under anesthesia with an invasive depth of up to 3 cm and no endoscopic-related complications. Endoscopically, the intestinal lesions of mice in dextran sodium sulfate group were gradually aggravated, but that were not changed in control group. Conclusion The endoscopic system can make clear imaging of intestinal mucosa and realize in vivo evaluation and dynamic monitoring of lesions.