Abstract:To explore the surgical technique and safety of totally robotic nephrectomy of living donation via retroperitoneal approach, and summarize its clinical experience. Methods To explore the surgical technique and safety of totally robotic nephrectomy of living donation via retroperitoneal approach, and summarize its clinical experience. Results All the 3 cases were completed successfully, no one was converted to open surgery. The operative time was 115.0 min, 120.0 min and 140.0 min respectively, blood loss in operation was 50 ml, 85 ml and 110 ml respectively. The donor kidney warm ischemic time was from 2.0~4.0 min, no operation-related complications occurred. All the drainage tubes were removed 3 d after operation, and all the donors discharged at 5 d after operation. Transplant kidneys urinated within 2.0 min after blood flow recovery, all transplant kidneys function reached to normal within one week. Conclusion Totally robotic nephrectomy for living donation via retroperitoneal approach is safe and feasible, with rapid postoperative recovery for donors, and no extra adverse effect on transplant kidneys. But the high cost of operation limits its extensive clinical application.