To investigate the safety and efficacy of laparoscopic ureteroureterostomy with intraoperative retrograde ureteroscopy-assisted technique in treatment of distal ureteral strictures and injuries. Methods The clinical data of 18 cases of ureteral distal ureteral strictures and injuries performed surgery from January 2016 to September 2017 were analyzed retrospectively. Results All the 18 operations were completed successfully. The operation time was (172.7 ± 22.5) min; the estimated blood loss was (132.8 ± 53.1) ml; the mean recovery time of gastrointestinal function after operation was (1.6 ± 0.4) d; the postoperative ambulation activity time was (1.4 ± 0.5) d; the mean hospital stay was (7.1 ± 1.7) d. After a period of (14.3 ± 4.9) months’ follow-up, no patient had ureter re-stenosis. Conclusion Laparoscopic ureteroureterostomy with intraoperative retrograde ureteroscopy-assisted technique for ureteral strictures and injuries is a safe, feasible, minimally invasive and accurate operation, which can accurate resection of stenosis/injury segment, and achieve in situ ureteral anastomosis without anatomical structural changes.