Abstract: Objective?To summarize the experience of the management and method in cardiopulmonary bypass (CPB) with mitral valve surgery by video-assisted thoracoscopy.?Methods?From January 2013 to June 2017, the clinical data of 80 patients underwent mitral vavle replacement by video-assisted thoracoscopic were reviewed and analyzed. Mild and moderate hypothermia were used in cardiopulmonary bypass (CPB). CPB cannulas of all the patients were established through femoral artery and vein. Vacuum-assisted venous drainage were implemented during the CPB. Myocardial protection of all the patients in operation was accomplished with HTK solution. The CPB established methods, CPB process and postoperative results of all the patients underwent operation by video-assisted thoracoscopy were analyzed.?Results?80 patients of the mitral valve disease were all recovered and discharged. The CPB time was 97?~?308 min (192.2?±?54.2)?min. The aortic cross-clamping time was 50~233?min (130.8?±?46.9)?min. The aortic declamping time was 30~53 min (41.8?±?8.1)?min. The post-operation respiratory auxiliary time was 6?~?24?h (20.6?±?14.1)?h. The post-operation ICU duration was 2?~?6 d (3.3?±?2.6)?d. The post-operation hospitalization duration was 7?~?10 d. All the patients recovered well and discharged uneventfully. And all the patients were followed up for 3?~?12 months. The clinical effects are satisfactory.?Conclusion?The CPB in the mitral valve surgery by video-assisted thoracoscopy is sate and feasible. But it should be modified to shorten the duration of CPB and aorta clamping. The various complications of intraoperation and post-operation should be avoided by the management of CPB.