内镜保胆胆囊组织结石复发易感基因调控研究

Integrating of the gene expression profiles in gallbladder stone and identify regulatory networks

摘要:

目的 采用高通量测序技术通过对胆囊结石复发患者胆囊黏膜中基因表达谱的检测及生物信 息学的分析，初步了解基因在胆囊结石发生发展中的作用机制。方法 用Illumina HiSeq 2500 新一代高通量 测序技术对7 例胆囊结石及2 例胆囊息肉复发患者进行mRNA 表达谱差异筛选分析，并对得到的数据进行 Gene Ontology（GO）和京都基因与基因组（KEGG）显著性富集统计。结果 高通量测序结果显示，差异 表达的mRNA 差异基因共有150 条。通过GO 功能显著性富集分析，发现分子功能的GO 功能显著富集是 抗原结合；而对于生物过程，富集的GO 功能是免疫应答；对于细胞成分，富集的GO 功能是细胞外区域。 KEGG 通路富集分析发现最显著的途径是细胞因子- 细胞因子受体作用途径（P =0.000）。结论 目前的研 究表明，研究胆囊结石复发易感基因，可以为预防内镜保胆术后胆囊结石复发的科学研究工作及临床治疗提 供一个重要的参考。

Abstract:

Objective The gene expression profile of gallbladder stone and gallbladder polyp were analyzed by high-throughput sequencing technology to explore the roles of the gene in the development of gallstone and their molecular mechanism in the occurrence and development of gallbladder stone. Methods 7 patients with gallstones and 2 patients with gallbladder polyp was enrolled for RNA-Seq. Results 150 DEGs was identified between gallstones and gallbladder polyps. We found GO terms for molecular functions significantly enriched in antigen binding, while for biological processes, the enriched GO terms were immune response, and for cellular component, the enriched GO terms were extracellular region. The most significant pathway in our KEGG analysis was Cytokinecytokine receptor interaction (P = 0.000). Conclusion This present study suggests some promising genes may provide a clue to the role of these genes played in the development of gallstones and gallbladder polyps.