支气管内超声引导针吸活检标本检测3种microRNA对肺癌分型的价值

Classification of lung cancer using three microRNAs in endobronchial ultrasound-guided tranbronchial needle aspiration samples

摘要:

目的??探讨支气管内超声引导针吸活检术（EBUS-TBNA）获取标本联合检测3种microRNA对肺癌分型的价值。方法?收集2014年10月-2016年1月在该院住院的肺癌患者35例，同期收集肺部良性病变者20例为对照组，经EBUS-TBNA获取标本，用实时定量PCR方法检测其miRNA-21、miRNA-205、miRNA-375的表达水平，评价其在肺癌病理分型的价值。结果?肺癌组EBUS-TBNA标本miRNA-21、miRNA-205和miRNA-375的相对表达量明显高于对照组，差异有统计学意义（均P <0.01）。miRNA-21在腺癌患者EBUS-TBNA标本中的表达水平明显高于肺鳞癌，差异有统计学意义（P <0.05）；miRNA-205在肺鳞癌患者EBUS-TBNA标本中的表达水平明显高于肺腺癌，差异有统计学意义（P <0.05）；miRNA-375在小细胞肺癌患者EBUS-TBNA标本中的表达水平明显高于鳞癌和腺癌，差异有统计学意义（P <0.01）。根据受试者工作特征曲线（ROC），miRNA-21取1.955为诊断肺腺癌的临界值时，其敏感度和特异度分别为65.00%和100.00%；miRNA-205取2.305为诊断肺鳞癌的临界值时，其敏感度和特异度均分别为91.67%和100.00%。结论?EBUS-TBNA标本行miRNA检测是可行的，联合检测miRNA-21、miRNA-205和miRNA-375有助于肺癌的诊断和病理分型。

Abstract:

Objective?To explore the significance of three microRNAs expression in endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) samples for classification of lung cancer. Methods?With qRT-PCR, the expression of miRNA-21, miRNA-205 and miRNA-375 were detected in EBUS-TBNA samples of 35 lung cancer patients. A group of 20 patients with benign mediastinal lymphadenopathy served as a control. The value of miRNA expressions for classification of lung cancer was evaluated. Results?The expressions of miRNA-21, miRNA-205 and miRNA-375 were significantly higher in EBUS-TBNA samples of lung cancer patients than those in control samples. The expressions of miRNA-21 were significantly higher in EBUS-TBNA samples of lung adenocarcinoma than those in lung squamous cell carcinoma. The expressions of miRNA-205 were significantly higher in EBUS-TBNA samples of lung squamous cell carcinoma than those in lung adenocarcinoma. The expressions of miRNA-375 were significantly higher in EBUS-TBNA samples of small cell lung cancer than those in non- small cell lung cancer. ROC curve analysis showed that taking 1.945 as a cutoff value, the sensitivity and specificity for miRNA-21 expression in EBUS-TBNA samples of lung adenocarcinoma were 65.00% and 100.00%; taking 2.305 as a cutoff value, the sensitivity and specificity for miRNA-205 expression in EBUS-TBNA samples of lung squamous cell carcinoma were 91.67% and 100.00%. Conclusion?EBUS-TBNA samples were suitable for detection of miRNA. Detecting miRNA-21, miRNA-205 and miRNA-375 expression could help diagnosis and classification of lung cancer.