Objective To discuss the technique and clinical effects of percutaneous transhepatic cholangioscopic lithotomy (PTCSL) in treatment of complicated biliary calculi. Methods Clinical data of 30 patients that used PTCSL to deal with complicated biliary calculi were retrospectively reviewed. The general data, clinical efficacy, postoperative complications and follow-up surveys were analyzed. Results All the patients were successfully performed PTCSL, there are no massive haemorrhage and death during and after operation. It is net extraction in 16 cases, without biliary leakage and bile peritonitis. Conclusion PTCSL in treatment of complicated biliary calculi is safe, with less perioperative complications. It worth of popularizing and applied clinically.