目的 探讨腹腔灌洗液中CK20mRNA的临床意义以及腹腔镜直肠癌根治术的临床应用价值。方法 选择49例直肠癌手术患者，利用RT-PCR技术分别检测以上患者腹腔灌洗液中CK20mRNA表达，从而探究CK20mRNA的临床价值以及评价腹腔镜直肠癌根治术的临床实用性。结果 10例良性病变腹部手术组患者腹腔灌洗液中CK20mRNA无明显表达。39例结直肠癌患者腹腔灌洗液中可见CK20mRNA表达，术前、术中以及术后的总表达率为40.2%（47/117），且术前、术中以及术后CK20mRNA的表达率比较差异无显著性（P >0.05）。17例开腹手术组和22例腹腔手术组术前、术中以及术后CK20mRNA阳性表达率的比较差异无显著性（P >0.05）。结论 腹腔镜结直肠癌根治术治疗结直肠癌安全有效，与开腹手术相比并不增加癌细胞局部种植的风险。
【Objective】 To investigate the clinical significance of the CK20mRNA in the peritoneal cavity washes, and the clinical value of the laparoscopic resection of colorectal cancer. 【Methods】 49 patients with colorectal cancer and conscience tumor were selected from our hospital . In order to explore the clinical value of the CK20mRNA and evaluate the practicality of laparoscopic resection of colorectal cancer, RT-PCR was used to detect the expression of CK20mRNA in the peritoneal cavity washes. 【Results】 The expressions of CK20mRNA were negative in the peritoneal cavity washes samples of 10 patients with non-malignant disease by operations on abdomen. Meanwhile, the total positive rate of expression of CK20mRNA were 40.2% (47/117) in the patients with colorectal carcinoma, and the expression of the CK20mRNA on pre-, during and post-operation didn't have significant difference (P >0.05). The positive expression rates of CK20mRNA in patients of the two groups (Laparoscopy group and Laparotomy group) pre-, during and post-operation didn't have significant difference (P >0.05). 【Conclusion】 Laparoscopic resection of colorectal cancer is safe and effective in the treatment of colorectal cancer. There are no significant differences in the efficacy of radical operation for patients with colorectal carcinoma by laparoscope and open radical surgery.